Figure 10. Knock-down of SITR protein in carp macrophages.

A. Western blot of cell lysates from carp macrophages incubated for 48 h with control morpholino (control, 5µM), SITR morpholino A (SITR_A, 5 µM) or SITR morpholino B (SITR B, 5 µM) or left untreated as control. SITR protein expression was analysed using anti-SITR antibody. B. Real-time gene expression in carp macrophages pre-incubated for 48 h with control morpholino (mo_ctrl, 5 µM), SITR morpholino A (mo_A, 5 µM) or SITR morpholino B (mo_B, 5 µM) or left untreated. Macrophages were further stimulated for 6 h with live T. borreli parasites (0.5×10⁶) or left unstimulated. mRNA levels of inducible nitric oxide synthase (iNOS) and Interleukin-1β (IL-1β) are shown relative to the house keeping gene 40S ribosomal protein S11 and are expressed as fold change relative to unstimulated cells (fold change = 1). Bars show averages ± SD of n = 4 fish. Symbol (*) shows a significant (P≤0.05) difference compared to macrophages incubated with control morpholino.