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. 2011 Jan 31;6(1):e16446. doi: 10.1371/journal.pone.0016446

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© 2011 Matsuo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A) Overall tyrosyl phosphorylation in WT, KO, D/A and K/R cells during differentiation. Total lysates were prepared from cells on days 0, 4 and 8 of differentiation. Blots were probed using anti-phosphotyrosine and anti-hPTP1B antibodies. Molecular weight markers are indicated on the right of the blot. Total lysates from cells at days 0, 4 and 8 of differentiation were immunoblotted for p-Erk (B) and p-Akt (C) and the corresponding proteins. (*) indicates statistically significant difference between KO, D/A or K/R and WT under the same treatment condition, while (^#) indicates statistically significant difference between WT, KO, D/A or K/R and their corresponding basal (day 0).