Table 3. Differentially expressed proteins in HIBM versus control muscle cultures.
| Ref. spot | Description and gene name | Swiss-Prot name/ID | HIBM/Control Fold change | Peptides m/o | Sequence coverage (%) | p-value | Mr (Da)a) | pI a) | Possible function |
| ID633952 | Myosin light chain 2; myosin regulatory light chain 2 | MLRS/Q96A32 | 0.28 | 5/64 | 7 | 2.5E-05 | 19014.7 | 4.91 | Cytoskeleton organization |
| ID633701 | Atrial/embryonic alkali myosin light chain | MYL4/P12829 | 0.44 | 21/103 | 49 | 0.030 | 21433.41 | 4.98 | Cytoskeleton organization |
| ID633133 | Tubulin alpha 6 | TBA1C/Q9BQE3 | 0.46 | 6/84 | 21 | 0.010 | 49895.6 | 4.96 | Cytoskeleton organization |
| ID633428 | Actin alpha cardiac muscle precursor | ACTC/P68032 | 2.96 | 12/83 | 26 | 0.031 | 42019.2 | 5.23 | Cytoskeleton organization |
| ID633599 | Tropomyosin 3 | TPM3/P06753 | 2.51 | 24/95 | 39 | 0.002 | 32818.79 | 4.68 | Cytoskeleton organization, cell motility |
| ID633361 | Vimentin | VIME/P08670 | 2.03 | 16/76 | 44 | 0.016 | 53686.0 | 5.06 | Cytoskeleton organization, cell motility |
| ID633085 | Radixin | RADI/P35241 | 0.34 | 6/64 | 12 | 0.048 | 68564.2 | 6.03 | Cytoskeleton organization, actin binding |
| ID633703 | Actin, gamma 2 propeptide | ACTH/P63267 | 2.37 | 8/84 | 9 | 0.020 | 41877.1 | 5.31 | Cytoskeleton organization, actin signaling |
| ID633154 | WD repeat-containing protein 1 isoform 2 | WDR1/O75083 | 0.28 | 5/61 | 7 | 0.018 | 58002.6 | 6.41 | Cytoskeleton organization, actin assembly |
| ID633113 | Kelch repeat and BTB (POZ) domain | KBTBA/O60662 | 0.46 | 14/75 | 25 | 0.010 | 66791.4 | 5.49 | Muscle contraction |
| ID633541 | Troponin T1 | TNNT1/P13805 | 0.25 | 8/82 | 9 | 0.004 | 32934.3 | 5.85 | Muscle contraction, calcium signaling |
| ID633032 | Nexilin | NEXN/Q0ZGT2 | 0.13 | 2/108 | 3 | 0.009 | 80658.28 | 5.31 | F actin binding, stress fibers |
| ID633067 | Zyxin | ZYX/Q15942 | 0.26 | 4/72 | 12 | 0.004 | 61277.7 | 6.22 | Cell adhesion, α- actinin binding protein |
| ID206244 | Proteasome 26S ATPase subunit 5 | PRS8/P62195 | 0.10 | 6/93 | 19 | 0.015 | 45626.3 | 7.11 | Ubiquitination pathway |
| ID633695 | Ubiquitin carboxyl-terminal esterase L1 | UCHL1/P09936 | 2.18 | 5/69 | 28 | 0.008 | 24824.5 | 5.33 | Ubiquitination pathway |
| ID633442 | COP9 signalosome subunit 4 | CSN4/Q9BT78 | 2.24 | 7/71 | 20 | 0.027 | 46269.1 | 5.57 | Ubiquitination pathway |
| ID633046 | Proteasome 26S non-ATPase subunit 2 | PSMD2/Q13200 | 0.47 | 13/87 | 19 | 0.039 | 100200.3 | 5.08 | Ubiquitination pathway, proteosome |
| ID633001 | Enolase 3 | ENOB/P13929 | 0.10 | 5/93 | 12 | 0.006 | 46959.1 | 7.58 | Glycolysis |
| ID633004 | 2-oxoglutarate dehydrogenase | ODO1/Q02218 | 0.20 | 12/59 | 10 | 0.001 | 113478.1 | 6.62 | Glycolysis |
| ID633347 | Enolase 2 | ENOG/P09104 | 2.29 | 4/76 | 5 | 0.015 | 47268.8 | 4.91 | Glycolysis |
| ID633294 | ATP synthase | ATPA/P25705 | 0.15 | 10/80 | 26 | 0.024 | 59750.9 | 9.16 | Oxidative phosphorylation |
| ID633089 | NADH dehydrogenase | NDUS1/P28331 | 0.26 | 11/89 | 23 | 0.004 | 79468.0 | 5.89 | Oxidative phosphorylation |
| ID633251 | Aldehyde dehydrogenase 1A1 | AL1A1/P00352 | 0.22 | 12/96 | 25 | 0.010 | 54862.1 | 6.30 | Electron transport, alcohol metabolism |
| ID633422 | Reticulocalbin 1 | RCN1/Q15293 | 2.29 | 7/61 | 25 | 0.041 | 38890.2 | 4.86 | Calcium binding |
| ID633408 | Reticulocalbin 3 | RCN3/Q96D15 | 3.25 | 8/92 | 41 | 0.010 | 37493.2 | 4.74 | Calcium binding, ER |
| ID633167 | Stress-induced-phosphoprotein 1 | STIP1/P31948 | 0.46 | 9/81 | 13 | 0.003 | 62639.6 | 6.40 | Stress response |
| ID633686 | Heat shock 27kD protein 1 | HSPB1/P04792 | 5.42 | 5/56 | 25 | 0.016 | 22782.6 | 5.98 | Stress response to unfolded proteins |
| ID633127 | Vesicle transport-related protein isoform a | SCFD1/Q8WVM8 | 0.29 | 6/72 | 16 | 0.003 | 72380.2 | 5.89 | Vesicle mediated transport, ER to Golgi |
| ID633030 | Coatomer protein complex, subunit gamma 1 | COPG/Q9Y678 | 0.17 | 11/73 | 14 | 6.9E-05 | 97718.8 | 5.32 | Vesicle mediated transport, Golgi to ER |
| ID633001 | RAN binding protein 5 | IPO5/O00410 | 0.38 | 6/82 | 5 | 0.006 | 125545.8 | 4.80 | Protein import to nucleus |
| ID633133 | Nuclear VCP-like | NVL/O15381 | 0.36 | 6/65 | 9 | 0.010 | 82746.6 | 6.04 | ATP binding triphosphatase activity |
| ID633294 | Glutamate dehydrogenase 1 | DHE3/P00367 | 0.15 | 7/80 | 16 | 0.024 | 61398.2 | 7.66 | AA metabolism |
| ID633154 | EH-domain containing 4 | EHD4/Q9H223 | 0.28 | 9/61 | 15 | 0.018 | 61175.5 | 6.33 | AA metabolism |
| ID633646 | 6-phospho- gluconolactonase | 6PGL/O95336 | 2.13 | 7/88 | 41 | 0.001 | 27547.0 | 5.70 | Carbohydrate metabolism |
| ID633646 | Peroxisomal long-chain acyl-coA thioesterase | ACOT2/P49753 | 0.10 | 6/93 | 15 | 0.001 | 53265.9 | 8.82 | Fatty acid metabolism |
| ID633236 | Dihydropyrimidinase-like 2 | DPYL2/Q16555 | 3.08 | 5/97 | 11 | 0.019 | 62294.0 | 5.95 | Cell communication |
| ID410685 | Cyclin-dependent kinase 3 | CDK3/Q00526 | 0.14 | 4/71 | 16 | 0.008 | 35045.9 | 8.86 | Cell cycle |
| ID633149 | Protein arginine methyltransferase 5 isoform a | ANM5/O14744 | 0.24 | 11/114 | 18 | 0.002 | 72684.3 | 5.88 | Cell proliferation |
| ID633341 | Lamin A/C | LMNA/P02545 | 0.25 | 11/54 | 21 | 0.017 | 65135.1 | 6.40 | Apoptosis signaling |
| ID633127 | PMF-1 binding protein | PMFBP/Q8TBY8 | 0.29 | 10/109 | 13 | 0.003 | 117480.4 | 5.90 | Cell death |
| ID633341 | Protein phosphatase 2 isoform b | 2AAB/P30154 | 0.46 | 7/75 | 11 | 0.017 | 73585.2 | 4.96 | Cell death |
Theoretical pI and Mr values were calculated using the ExPASY tool. m = matched peptides, o = observed peptides.