Table 4. Differentially expressed proteins in HIBM versus control muscle biopsies as determined by 2-DE analysis.
| Ref. spot | Description and gene name | Swiss-Prot name/ID | HIBM/Control Fold change | Sequence coverage (%) | Theoretical Mr (Da)a) | Theoretical pI a) | No. of confirmatory MS/MS peptides | Possible function |
| 1206 | Slow skeletal ventricular myosin alkali light chain 3 | MYL3/P08590 | 4.95 | 78 | 21800.87 | 5.03 | 19 | Cytoskeleton organization |
| 3515 | Myosin heavy chain 7 cardiac muscle, beta | MYH7/P12883 | 2.90 | 25 | 223733.72 | 5.60 | 41 | Cytoskeleton organization |
| 1401 | Actin alpha cardiac muscle precursor | ACTC/P68032 | 2.01 | 53 | 41784.64 | 5.23 | 12 | Cytoskeleton organization |
| 1211 | Myosin light chain 2 | MLRS/Q96A32 | 1.28 | 86 | 18883.38 | 4.91 | 13 | Cytoskeleton organization |
| 3511 | Vinculin isoform VCL | VINC/P18206 | 1.92 | 65 | 123668.11 | 5.51 | 63 | Cytoskeleton organization |
| 1401 | Tropomyosin 1 alpha chain isoform 1 | TPM1/P09493 | 7.43 | 87 | 32708.57 | 4.69 | 48 | Cytoskeleton organization |
| 4407 | Moesin | MOES/P26038 | 15.27 | 39 | 67688.85 | 6.09 | 25 | Cytoskeleton organization |
| 7404 | Myotilin | MYOTI/Q9UBF9 | 3.92 | 44 | 55395.14 | 9.12 | 20 | Cytoskeleton organization |
| 4403 | Radixin | RADI/P35241 | 1.10 | 42 | 68563.90 | 6.03 | 23 | Cytoskeleton organization |
| 8204 | Troponin I, skeletal, slow | TNNI1/P19237 | 46.12 | 49 | 21561.15 | 9.61 | 13 | Muscle contraction |
| 3305 | Troponin T, slow skeletal muscle | TNNT1/P13805 | 1.45 | 36 | 32816.97 | 5.86 | 15 | Muscle contraction |
| 7240 | Troponin I, skeletal, fast | TNNI2/P48788 | 0.69 | 58 | 21207.33 | 8.88 | 16 | Muscle contraction |
| 2412 | Heat shock 70kDa protein 8 isoform 1 | HSP7C/P11142 | 1.60 | 61 | 70766.90 | 5.37 | 41 | Stress response to unfolded proteins |
| 3204 | Heat shock 27kDa protein1 | HSPB1/P04792 | 1.49 | 47 | 22782.52 | 5.98 | 11 | Stress response to unfolded proteins |
| 5208 | Crystallin, alpha B | CRYAB/P02511 | 1.22 | 71 | 20158.91 | 6.76 | 15 | Stress response to unfolded proteins |
| 7401 | ATP synthase, H+ transporting, mitochondrial | ATPA/P25705 | 1.83 | 51 | 55209.32 | 8.28 | 32 | Oxidative phosphorylation |
| 2408 | NADH dehydrogenase (ubiquinone) Fe-S protein1 | NDUS1/P28331 | 2.26 | 53 | 79467.50 | 5.89 | 30 | Oxidative phosphorylation |
| 2305 | Ubiquinol-cytochrome c Reductase core protein I | QCR1/P31930 | 2.20 | 48 | 52645.82 | 5.94 | 21 | Oxidative phosphorylation |
| 2312 | Cytosolic malate dehydrogenase | MDHC/P40925 | 0.51 | 33 | 36294.93 | 6.89 | 10 | Carbohydrate metabolism |
| 5516 | Aconitase 2 | ACON/Q99798 | 2.35 | 40 | 85425.41 | 7.36 | 27 | Carbohydrate metabolism |
| 4505 | Oxoglutarate dehydrogenase isoform | ODO1/Q02218 | 1.74 | 44 | 115935.28 | 6.39 | 42 | Glycolysis |
| 6409 | Pyruvate kinase | KPYM/P14618 | 1.25 | 73 | 57805.70 | 7.95 | 44 | Glycolysis |
| 6513 | Phosphofructokinase, muscle | K6PF/P08237 | 3.68 | 21 | 85051.33 | 8.23 | 14 | Glycolysis |
| 5505 | Adenosine monophosphate deaminase 1 | AMPD1/P23109 | 2.20 | 58 | 86489.87 | 6.43 | 38 | Purine metabolism |
| 6208 | Prostatic binding protein | PEBP1/P30086 | 4.40 | 57 | 20925.59 | 7.43 | 9 | Signal transduction in nervous system |
| 5210 | Beta globin | HBB/P68871 | 0.60 | 96 | 15867.22 | 6.81 | 14 | Hemoglobin polymerization |
a)
Theoretical pI and Mr values were calculated using the ExPASY tool.