Table 6. Differentially expressed proteins in HIBM versus control muscle biopsies as determined by iTRAQ analysis.
| Description and gene name | Swiss-Prot name/ID | HIBM/Control Fold change | Sequence coverage (%) | Theoretical Mr (Da)a) | Theoretical pI a) | No. of confirmatory MS/MS peptides | Possible function |
| Myosin light polypeptide 6B | MYL6B/P14649 | 0.53 | 51 | 22763.99 | 5.56 | 11 | Cytoskeleton organization |
| Myosin heavy chain 3 | MYH3/P11055 | 0.70 | 19 | 223904.73 | 5.62 | 3 | Cytoskeleton organization |
| Myosin heavy chain 2 | MYH2/Q9UKX2 | 3.08 | 57 | 223044.46 | 5.64 | 68 | Cytoskeleton organization |
| Myosin light chain 2 | MLRS/Q96A32 | 2.2 | 88 | 18883.38 | 4.91 | 23 | Cytoskeleton organization |
| Myosin heavy chain 4 | MYH4/Q9Y623 | 1.63 | 33 | 223071.41 | 5.65 | 6 | Cytoskeleton organization |
| Alpha-actinin-2 | ACTN2/P35609 | 1.28 | 61 | 103853.79 | 5.31 | 62 | Cytoskeleton organization |
| Tropomyosin alpha-1 chain | TPM1/P09493 | 2.96 | 54 | 32708.57 | 4.62 | 17 | Cytoskeleton organization |
| Tropomyosin beta chain | TPM2/P07951 | 1.72 | 60 | 32850.73 | 4.66 | 19 | Cytoskeleton organization |
| Myomesin-2 | MYOM2/P54296 | 1.32 | 15 | 164896.31 | 5.82 | 17 | Cytoskeleton organization |
| Tubulin beta chain | TBB5/P07437 | 1.49 | 11 | 49670.82 | 4.78 | 4 | Cytoskeleton organization |
| Troponin C, skeletal muscle fast | TNNC2/P02585 | 2.06 | 45 | 17990.86 | 4.06 | 7 | Muscle contraction |
| Troponin I, fast skeletal muscle | TNNI2/P48788 | 2.33 | 33 | 21207.33 | 8.88 | 12 | Muscle contraction |
| Ankyrin repeat domain-containing protein 2 | ANKR2/Q9GZV1 | 1.12 | 23 | 39859.25 | 5.72 | 6 | Muscle stress response |
| Sarcalumenin | SRCA/Q86TD4 | 1.20 | 8 | 48692.49 | 3.81 | 5 | Calcium transport |
| Protein S100-A4 | S10A4/P26447 | 0.74 | 27 | 11597.32 | 5.88 | 4 | Tubulin Polymerization |
| Pyruvate kinase isozymes M1/M2 | KPYM/P14618 | 1.58 | 55 | 57805.70 | 7.95 | 26 | Glycolysis |
| Gamma-enolase | ENOG/P09104 | 1.62 | 19 | 47137.39 | 4.91 | 1 | Glycolysis |
| Glucose-6-phosphate isomerase | G6PI/P06744 | 1.50 | 9 | 63015.93 | 8.44 | 5 | Glycolysis |
| Alpha-enolase | ENOA/P06733 | 1.12 | 24 | 47037.77 | 6.99 | 4 | Glycolysis |
| Triosephosphate isomerase | TPIS/P60174 | 1.38 | 85 | 26538.30 | 6.51 | 19 | Glycolysis |
| Malate dehydrogenase, mitochondrial precursor | MDHM/P40926 | 1.06 | 29 | 35503.28 | 8.92 | 9 | Carbohydrate metabolism |
| Malate dehydrogenase, cytoplasmic | MDHC/P40925 | 0.91 | 41 | 36294.93 | 6.89 | 12 | Carbohydrate metabolism |
| Phosphoglucomutase-1 | PGM1/P36871 | 1.68 | 23 | 61317.93 | 6.32 | 13 | Carbohydrate metabolism |
| Glycogen debranching enzyme | GDE/P35573 | 1.64 | 11 | 174763.74 | 6.31 | 13 | Carbohydrate metabolism |
| Aldose reductase | ALDR/P15121 | 1.18 | 16 | 35722.21 | 6.55 | 5 | Carbohydrate metabolism |
| Glycogen phosphorylase | P11217/PYGM | 1.78 | 59 | 96960.86 | 6.57 | 48 | Carbohydrate metabolism |
| Glyceraldehyde-3-phosphate dehydrogenase | G3P/P04406 | 1.42 | 66 | 35922.02 | 8.58 | 34 | Carbohydrate metabolism |
| Cytochrome c oxidase subunit 4 | COX41/P13073 | 1.11 | 24 | 19576.71 | 9.52 | 4 | Oxidative phosphorylation |
| Calsequestrin-1 | CASQ1/P31415 | 3.31 | 21 | 41688.88 | 3.96 | 6 | Mitochondrial calcium binding |
| Dihydrolipoyl dehydrogenase | DLDH/P09622 | 0.85 | 9 | 54177.25 | 7.95 | 3 | Mitochondrial glycine cleavage |
| Hydroxyacyl-coenzyme A dehydrogenase, | HCDH/Q16836 | 0.87 | 11 | 34277.50 | 8.88 | 3 | Mitochondrial oxidation |
| Cytochrome b-c1 complex | UCRI/P47985 | 1.37 | 19 | 29667.99 | 8.55 | 3 | Mitochondrial oxidation |
| Peroxiredoxin-6 | PRDX6/P30041 | 1.45 | 34 | 24903.79 | 6.02 | 7 | Redox regulation |
| Histidine triad nucleotide-binding protein 1 | HINT1/P49773 | 1.13 | 51 | 13670.72 | 6.46 | 4 | Nucleotide binding |
| Probable C→U-editing enzyme APOBEC-2 | ABEC2/Q9Y235 | 1.53 | 46 | 25703.14 | 4.81 | 7 | mRNA editing |
| Phosphatidylethanolamine-binding protein 1 | PEBP1/P30086 | 1.27 | 42 | 20925.59 | 7.43 | 5 | Signal transduction in nervous system |
| Acyl-CoA-binding protein | ACBP/P07108 | 1.11 | 20 | 9913.23 | 6.11 | 2 | Lipid metabolism |
| Serum albumin precursor | ALBU/P02768 | 1.49 | 68 | 66472.21 | 5.67 | 54 | Fatty acid & steroid transport |
| Glycerol-3-phosphate dehydrogenase | GPDA/P21695 | 1.69 | 20 | 37436.44 | 5.82 | 6 | Glycerol metabolism |
| Alpha-1-antitrypsin precursor | A1AT/P01009 | 0.78 | 20 | 46736.55 | 5.37 | 7 | Protease inhibition |
| Aspartate aminotransferase, mitochondrial | AATM/P00505 | 1.43 | 25 | 47475.57 | 9.14 | 9 | AA metabolism |
a)
Theoretical pI and Mr values were calculated using the ExPASY tool.