Figure 2. SDS-PAGE showing recombinant DBL and CIDR domains from ITvar9 expressed in E. coli.

The purity and quality of the recombinant DBL and CIDR domains were assessed by electrophoresis of reduced and non-reduced pairs of proteins on 10% SDS-polyacrylamide gels. Two µg of protein was used per well and lanes were as follows: 1) NTS-DBL1α, 2) DBL1α, 3) DBL2γ 4) DBL3ε, 5) DBL4δ, 6) CIDR2β and 7) NTS-DBL1α-CIDR1γ. M, molecular weight marker; NR, non-reduced; R, reduced.