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. 2011 Jan 31;6(1):e16414. doi: 10.1371/journal.pone.0016414

Figure 3. Immunofluorescence assay showing that ITvar9 antibodies recognize PfEMP1 on the surface of live infected erythrocytes.

Figure 3

R29 mature infected erythrocytes (pigmented trophozoites and schizonts) were grown to 5% parasitaemia and incubated with rabbit antisera against recombinant ITvar9 DBL and CIDR domains at 1/50 dilution. After washing, the cells were incubated with Alexa Fluor 488-labelled goat anti-rabbit IgG (Invitrogen) at 1/1000 dilution. The example shown here is the binding of anti-DBL2γ antisera, however all antisera to ITvar9 gave similar results. Punctate staining of the membrane of infected erythrocytes (green) was seen with the specific antisera (“immune”) but not with the pre-immune sera. The location of infected erythrocytes is shown by DAPI staining of the parasite (blue). Slides were viewed with a 100× objective using a Leica DM 2000 fluorescent microscope.