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. 2011 Jan 31;6(1):e16414. doi: 10.1371/journal.pone.0016414

Figure 5. ELISA to detect binding of PfEMP1 antibodies to recombinant NTS-DBL1α.

Figure 5

Antibodies were added to wells coated with 2 µg/ml of recombinant NTS-DBL1α protein and binding was detected using HRP-conjugated anti-rabbit IgG at 1/10,000 dilution. Antisera raised to recombinant proteins containing DBL1α (i.e. anti-NTS-DBL1α, anti-DBL1α and anti-NTS-DBL1α-CIDR1γ) all recognize the recombinant protein as expected. Antisera to DBL2γ, DBL3ε and CIDR2β do not cross-react with recombinant NTS-DBL1α. However, the antiserum to DBL4δ does shows binding to the recombinant NTS-DBL1α, suggesting that there is cross-reactivity between these two domains.