Table 1. Effectiveness of ITvar9 antibodies in various assays^#.

Antibodies	IFA end titre$ (µg/ml)	Rosette Inhibition IC50* (µg/ml)	Phagocytosis ∼50% of positive control (µg/ml)
Negative control rabbit IgG	Negative at 100	Negative at 500	Not done
Anti-NTS-DBL1α	0.024 †	0.04	<1.56
Anti-DBL1α	0.39	0.1	6.25
Anti-NTS-DBL1α-CIDR1γ	0.10	0.05	1.56
Anti-DBL2γ	0.39	>500	100
Anti-DBL3ε	0.10	2	1.56
Anti-DBL4δ	0.10	1	6.25
Anti-CIDR2β	1.56	4	6.25

^#50% ELISA titres for the antibodies (defined as the titre giving 50% of the maximum OD) were as follows: NTS-DBL1α 1/500,000; DBL1α 1/250,000; NTS-DBL1α-CIDR1γ 1/300,000; DBL2γ 1/50,000; DBL3ε 1/200,000; DBL4δ 1/40,000 and CIDR2β 1/200,000.

^$The lowest concentration at which >50% of the infected erythrocytes in the culture showed punctate fluorescence by IFA. Values shown are µg/ml of purified total IgG.

*50% inhibitory concentration (IC50) for rosette inhibition. Values shown are µg/ml of purified total IgG.

^†The most effective antibodies in each assay are shown in bold. Values shown are µg/ml of purified total IgG.