Figure 6. Nanog shRNA enhances the inhibitory effects of resveratrol on self-renewal capacity of pancreatic cancer stem cells.
(A), Expression of Nanog. Pancreatic CSCs were transduced with scrambled or Nanog shRNA, and Western blot analyses were performed to examine the expression of Nanog and GAPDH. (B), Transduced cells were treated with resveratrol (0–30 µM) and spheres in suspension were grown for one week. Spheres were harvested, resuspended and cell viability was determined by trypan blue assay.