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A. Identification of an insertion mutant by PCR using primers Cac1494B and Pex1494E flanking the target site; B. Identification of the deletion mutants by PCR using primers P1492-5s and Pex1494E; C. Schematic show of operon CAC1493-1494 and the expected deleted operon CAC1493-1494 in the chromosome; D. Southern blot analysis of CAC1493-1494 deletion using CAC34 probe.
