Table 1. Bacterial strains, plasmids and primers.
| Strains, plasmids or primers | Relevant characteristics | Reference or source |
| Strains | ||
| E. coli Top10 | mcrA Δ(mrr-hsdRMS-mcrBC) recA1 | Invitrogen |
| E. coli JM109 | recA1 mcrB+ hsdR17 | Lab storage |
| C. acetobutylicum DSM1731 | Contains operon CAC1493-1494, wild type | DSMZ |
| C. acetobutylicum DC1494 | CAC1494::intron with H1 fragment | This study |
| C. acetobutylicum DDC14 | ΔCAC1493-1494 | This study |
| C. acetobutylicum DDC245 | ΔCAC1493-1494 | This study |
| C. acetobutylicum DCctfb3 | ctfb::intron with H2 fragment | This study |
| C. acetobutylicum DCctfb7 | ctfB::intron with H2 fragment | This study |
| C. acetobutylicum DDC1458 | ΔctfAB | This study |
| Plasmids | ||
| pMTL007 | Cmr, ClosTron | [24] |
| pMTL007-1494 | Derived from pMTL007, targeting the CAC1494 in C. acetobutylicum | This study |
| pAN2 | Φ3t1, p15a ori, Tetr, methylating DNA prior to transformation to protect it against a C. acetobutylicum restriction system | [24] |
| pMD18T-simple | Ampr | Takara |
| pMD18T-007 | pMD18T-simple ligated with Ll.ltrB intron | This study |
| pMD18T-007H1 | pMD18T-007 ligated with H1 fragment | This study |
| pMTL007-H1 | pMTL007-1494 containing H1 fragment, CAC1493-1494 deletion vector | This study |
| pMTL007-ctfb | Derived from pMTL007, targeting the ctfB in C. acetobutylicum | This study |
| pMTL007-H2 | pMTL007-ctfb containing H2 fragment, ctfAB deletion vector | This study |
| Primer | ||
| 5402F-F1 | 5′-TTAAGGAGGTGTATTTCATATGACCATGATTACG-3′ | [24] |
| Re-3204 | 5′-TTCAGGTGTTATTCTTTCTGGACTTTCTCGGT-3′ | This study |
| 1494-385/386s-IBS | 5′-AAAAAAGCTTATAATTATCCTTAATGGTCTATATCGTGCGCCCAGATAGGGTG-3′ | This study |
| 1494-385/386s-EBS1d | 5′-CAGATTGTACAAATGTGGTGATAACAGATAAGTCTATATCATTAACTTACCTTTCTTTGT-3′ | This study |
| 1494-385/386s-EBS2 | 5′-TGAACGCAAGTTTCTAATTTCGGTTTCCTTTCGATAGAGGAAAGTGTCT-3′ | This study |
| Cac1494B | 5′-CGCGGATCCTTGTGTAAGCACATTTTAGG-3′ | This study |
| Pex1494E | 5′-CCGGAATTCTTATACACATATTGGCTCTC-3′ | This study |
| P1492-5s | 5′-ACGCGTCGACGCTGGTGCTTTACTTGAACT-3′ | This study |
| Clos-5 | 5′-AAAACACGTGATATGGCTAAACCTCCCAAG-3′ | This study |
| Clos-3 | 5′-ACGCCACGTGAAACTTGCCCTTTCCTATTC-3′ | This study |
| Sp5 | 5′-AATGGTGCTGCAACAAAATATATT-3′ | This study |
| Sp3 | 5′-CATCTTGATTAATAAATTCTACAT-3′ | This study |
| CTFB572/573s-IBS | AAAAAAGCTTATAATTATCCTTATTACTTCTCACTGTGCGCCCAGATAGGGTG | This study |
| CTFB572/573s -EBS1d | CAGATTGTACAAATGTGGTGATAACAGATAAGTCCTCACTGATAACTTACCTTTCTTTGT | This study |
| CTFB572/573s -EBS2 | TGAACGCAAGTTTCTAATTTCGGTTAGTAATCGATAGAGGAAAGTGTCT | This study |
| Dadhe-5 | AAAACACGTGTCAGAACACAATATTCCTAG | This study |
| Dad-3 | ACGCCACGTGCAATCATAATTGTCATCCCA | This study |
| Ct-5 | AGCCAATTGGATTGTTCCTG | This study |
| CTFB-3 | CAGCCATGGGTCTAAGTTCA | This study |
| Pro2-5 | ACTAGATGATCAATGCACAG | This study |
| Pr2-3 | GAGATTGTTTCTAGCTCTCA | This study |
Abbreviations: Ampr, ampicillin resistance; Cmr, chloramphenicol resistance; Tetr, tetracycline resistance; Φ3t1, Φ3t1 methyltransferase gene of Bacillus subtilis phage Φ3t1. DSMZ, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.