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. 2011 Jan 31;6(1):e14629. doi: 10.1371/journal.pone.0014629

Figure 4. Detection of PPARα binding on the two CYP1A1 PPRE sites by Gel shift assay.

Figure 4

A PPRE canonical sequence, the CYP1A1 PPRE1 (position –931/-919) and the CYP1A1 PPRE2 (position –519/-531) were tested. 1: TNT + PPRE canonical sequence; 2: TNT + RXR + PPRE canonical sequence; 3: TNT + RXR + PPARα + PPRE canonical sequence; 4: TNT + RXR + PPARγ + PPRE canonical sequence; 5: TNT + PPRE 1; 6: TNT + RXR + PPRE 1; 7: TNT + RXR + PPARα + PPRE 1; 8: TNT + RXR + PPARγ + PPRE 1; 9: TNT + PPRE2; 10: TNT + RXR + PPRE2; 11: TNT + RXR + PPARα + PPRE2; 12: TNT + RXR + PPARγ + PPRE2.