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. Author manuscript; available in PMC: 2012 Mar 1.

Published in final edited form as: Acta Biomater. 2010 Nov 11;7(3):1249–1264. doi: 10.1016/j.actbio.2010.11.007

Quantitative RT-PCR analysis of gene expression profiles of growth factors (A: BMP-2, B: FGF-2, C: TFG- β1, and D: Runx2) for 1, 4, and 8 days. The fold changes in gene expression level are reported as average ± standard deviation (n=3). The calibrator for all experimental groups is indicated by a Δ marker and dashed line indicates the fold change of the calibrator group. + indicates a statistical difference in HA amount within the same cell seeding density group as compared to the 0% HA control group while # indicates a statistical difference in cell seeding density groups within the same HA concentration group (p<0.05).

Quantitative RT-PCR analysis of gene expression profiles of growth factors (A: BMP-2, B: FGF-2, C: TFG- β1, and D: Runx2) for 1, 4, and 8 days. The fold changes in gene expression level are reported as average ± standard deviation (n=3). The calibrator for all experimental groups is indicated by a Δ marker and dashed line indicates the fold change of the calibrator group. + indicates a statistical difference in HA amount within the same cell seeding density group as compared to the 0% HA control group while # indicates a statistical difference in cell seeding density groups within the same HA concentration group (p<0.05).