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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Brain Behav Immun. 2010 Sep 18;25(5):872–882. doi: 10.1016/j.bbi.2010.09.012

Fig. 6. Dietary DHA inhibits Th1 and Th17 differentiation and reduces the numbers of CNS infiltrating Th1, Th17 and total CD4+ T cells.

Fig. 6

C57BL/6 mice fed a control or DHA diet were immunized with MOG35–55 as described in Methods. (A) Splenocytes (2×106/ml) isolated from spleen on day 11 post immunization (before disease onset) were cultured ex vivo in the presence of 50 µg MOG35–55 for 3 days. Proliferation was determined by [3H]-thymidine incorporation. Supernatants were subjected to ELISA for IL-17 and IFNγ. One representative experiment out of three is shown. (B–C) Intracellular cytokine staining of FITC-gated CD4+ T cells isolated from brain (B) or spinal cord (C) of mice at peak of disease (day 18) (cells pooled from 3 animals) (left panels show flow cytometric analysis; right panels show calculated numbers of cytokine-producing cells). Data are representative of two independent experiments.