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. Author manuscript; available in PMC: 2012 Jan 28.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Dec 25;404(4):979–984. doi: 10.1016/j.bbrc.2010.12.095

Fig. 2.

Fig. 2

The C-terminal PDZ binding motif of TRPV5 is required for the effects of NHERF2 on TRPV5 and the regulation of WNK4 on TRPV5. A. The stimulation effect of NHERF2 on TRPV5 was abolished in the TRPV5-C6 construct which has the sequence of TRPV5 with the last 2 amino-acids substituted by those of TRPV6 (n = 14). The PDZ binding motifs of TRPV5 and TRPV6 are shown in the upper panel. B. The effect of NHERF2 on the regulation of TRPV5 by WNK4 was abolished in the TRPV5-C6 construct; however, substituting the last 2 amino-acids of TRPV6 with those of TRPV5 (TRPV6-C5) was not sufficient to make it responsive to WNK4 (n = 14). NHERF2 was co-expressed in all groups. NS, not statistically significant (P > 0.05). Representative Western blot analyses using anti-TRPV5 or anti-TRPV6 antibody are shown in the lower panel.