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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Microbes Infect. 2010 Nov 9;13(3):232–238. doi: 10.1016/j.micinf.2010.10.021

Fig 5.

Fig 5

Real time PCR analysis of E. chaffeensis inhibition of nuclear and mitochondrial gene transcription. RNA was extracted from DH82 cells harvested at 24, 48, 72, and 96 hours after E. chaffeensis infection and used as template for reverse transcription. The cDNA was amplified by real time PCR using primers from the nuclear GAPDH gene and mitochondrial genes nadph2 (ND) and cyt b. The relevant transcriptional level of mitochondrial gene were calculated by the transcriptional level of mitochondrial gene/ the transcriptional level of nuclear gene GAPDH. * indicating significant difference of transcriptional levels between mitochondrial NADPH or Cyt b gene and nuclear GAPDH gene.