. Author manuscript; available in PMC: 2012 Mar 15.

Published in final edited form as: Anal Biochem. 2010 Nov 26;410(2):248–256. doi: 10.1016/j.ab.2010.11.034

Table I.

Glycated peptides identified by LC/ESI/MS and LC/ESI/MS/MS

Tryptic Digest Fragments	Peptide _aa	Theoretical Mass of Fragments in Da	LC/ESI/MS Observed Mass in Da (charge state)	Δ m/z	Analysis by Tandem MS Incorporating CID
FK^*DLGEENFK	11–20	1226.6	1391(+2)	164	¹²K
AEFAEVSK^*LVTDLTK	226–240	1650.9	1815(+2)	164	²³³K
LK^ECCEK^PLLEK	275–286	1432.8	1597(+2)	164	²⁷⁶ K or ²⁸¹K
K^*VPQVSTPTLVEVSR	414–428	1639.9	1804(+2)	164	⁴¹⁴K
K^*QTALVELVK	525–534	1128.7	1293(+2)	164	⁵²⁵K

Galactated HSA was prepared by incubating the native protein (240 mg) with D-galactose (1.85 M) in 3 ml of 50 mM phosphate buffer, pH 7.4, for 2h at 56°C.

Denotes that these sites represent galactated lysine residues