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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: J Cell Biochem. 2011 Jan;112(1):39–48. doi: 10.1002/jcb.22710

Figure 1.

Figure 1

PKCδ is a substrate for PTPμ. A549 cells were treated with (+) or without (−) 100 μM pervanadate (PV) for 20 minutes. Cells were lysed and equal amounts of protein were incubated with iPTPμWT-ΔD2-GST, iPTPμDA-ΔD2-GST or GST immobilized on glutathione Sepharose. Associated proteins were resolved by SDS-PAGE (8% for PKCδ and 10% for RACK1) and detected by immunoblot. PKCδ and RACK1 were detected in all the PTPμ pull downs. Phospho-PKCδ Y311 was detected only in the iPTPμDA-ΔD2-GST pull down, indicating PKCδ is a PTPμ substrate. The RACK1 immunoblot was stripped and probed with an antibody directed against GST to show the relative amounts of GST fusion proteins used.