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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: J Cell Biochem. 2011 Jan;112(1):39–48. doi: 10.1002/jcb.22710

Figure 2.

Figure 2

PLCγ1 is a substrate for PTPμ and interacts with PTPμ in cells. A549 cells were treated with (+) or without (−) 100 μM pervanadate (PV) for 20 minutes. Cells were lysed and equal amounts of protein were incubated with iPTPμWT-ΔD2-GST, iPTPμDA-ΔD2-GST or GST immobilized on glutathione Sepharose. Associated proteins were resolved by SDS-PAGE (6%) and detected by immunoblot. PLCγ1 was detected in all the PTPμ pull downs but phospho-PLCγ1 (Y783) was detected only in the iPTPμDA-ΔD2-GST pull down, and dephosphorylated by iPTPμWT-ΔD2 indicating that PLCγ1 is a PTPμ substrate (A). Confluent A549 cells were treated with the DSP cross-linking agent. Immunoprecipitations were performed using a polyclonal antibody to an extracellular epitope of PTPμ or non-immune rabbit serum (NIRS). Proteins were resolved by SDS-PAGE (6%) and immunoblotted for PTPμ and PLCγ1 (B).