Figure 3. Alternative methods for targeted RNA-seq.
a | Using poly(A)+ RNA converted to double-stranded cDNA the Agilent SureSelect method uses RNA probes to enrich selected cDNA62. b | A custom NimbleGen array may allow selection of cDNAs of interest. c | The generation of DNA molecules with sequence-specific complementary targeting sites allows the targeting of cDNAs63,64. d | Helicos sequencing surfaces containing target-specific oligonucleotides can be used to select desired RNA, DNA and cDNA species and sequence regions of interest in a single step. nt, nucleotide.