Table 1.
TSS method | RNA sequence data | starting RNA | Refs |
---|---|---|---|
CAGE | 5′ end of transcripts | 50 μg total RNA | 9 |
DeepCAGE | 5′ end of transcripts | 10 ng total RNA | 10 |
nanoCAGE | 5′ end of transcripts | 10 ng total RNA | 12 |
CAGEscan | 5′ end of transcripts and either 3′ end or internal RNA sequence | 10 ng total RNA | 12 |
PEAT | 5′ end of transcripts paired with random reads along the RNA | 150 μg total RNA | 11 |
CAGE, cap analysis of gene expression; CAGEscan, paired read to combine 5′ CAGE with downstream sequence; DeepCAGE, high-throughput CAGE sequencing; nanoCAGE, low-quantity CAGE; ng, nanograms; PEAT, paired end analysis of transcription start sites; TSS, transcription start site.