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. 2010 Dec 7;104(2):324–331. doi: 10.1038/sj.bjc.6606026

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Copyright © 2011 Cancer Research UK

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Expression of HPV16 E6 is associated with the upregulation of GEF16 and higher levels of activated Cdc42 in non-transformed cells. (A) Competitive template RT–PCR analysis of GEF16 levels in mRNAs, extracted from hTertV and hTertT16 E6 cells. mRNA was extracted, reverse transcribed and the resulting cDNAs amplified with primers specific for β-actin, HPV16 E6 and competitive template primers specific for GEF16. (B) Western immunoblot of proteins extracted from hTert cells stably transfected with either HPV16 E6, HPV6 E6 or vector control. β-Actin was used as a loading control. (C) Western immunoblot analysis of GTP-activated Cdc42 in HPV16 E6- and HPV6 E6-transfected hTert cells. hTertT16 E6 cells showed increased levels of activated Cdc42. The addition of GTP to the PAK1 Cdc42 assay shows constitutive GTP activation of the available Cdc42.