Table 1. S1 and S4 gene mutations of persistently infecting reoviruses.

		Mutation position
Persistent reovirus	Reovirus gene	Nucleotide	Amino acid	GenBank accession no.
HTR1	S1	359, T–C	116, L–P	AY860061
		392, T–C	127, V–A
		763, C–T	251, Q–Stop
		912, A–G	300, I–M
	S4	562, C–T	177, S–F	DQ915165
		784, A–T	251, H–L
CA46 PI	S1	89, A–C	26, K–T	EF133509
			7, K–Q
		1351, A–T	447, T–S
	S4	720, C–T	230, H–Y	EF133510
		730, C–T	233, S–L
		1089, A–C	353, N–Q
		1091, T–G	353, N–Q
		1092, T–C	354, Y–H
Raji PI	S1	—	—	EF133511
	S4	784, A–T	251, H–L	EF133512

Mutations in S1 and S4 gene nucleotide sequences of persistently infecting reoviruses from HTR1, Raji PI and CA46 PI cells, and corresponding mutations in the deduced amino acid sequences of their gene products (S1: σ1/σ1 s, S4: σ3) compared with previously published sequences of WT reovirus (GenBank accession no: S1 (X01161), S4 (K02739)). As reovirus occasionally undergoes spontaneous genome variations, nucleotide changes that were found in all the persistently infecting reoviruses and in our lab, WT reovirus were not included in this table (S1: 366, C–G, 367, G–C, S4: 624, G–A, 719, G–T).