Skip to main content
. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Mitochondrion. 2006 Aug 3;6(5):235–244. doi: 10.1016/j.mito.2006.07.008

Fig. 3.

Fig. 3

Identification of ROS-generating site in 3-NPA-treated mitochondria. (A) 3-NPA inhibits ROS generation from A549 or MH-S mitochondria respiring on the complex II substrate, succinate. Purified mitochondria respiring on succinate were exposed to 3-NPA (3 mM, pH 7.4) and O2•− levels were determined after its dismutation to H2O2 by SOD by Amplex Red assays. MT, mock-treated; AA, Antimycin A; CCCP, carbonylcyanide m-chlorophenyl-hydrazone. **p < 0.01, ***p < 0.001, ****p < 0.0001. (B) 3-NPA increases ROS production from A549 mitochondria in the presence of pyruate + malate. Changes in O2•− levels were determined as in A. ***p < 0.001, ****p < 0.0001. MT, mock-treated; antimycin A, AA; SOD, superoxide dismutase. (C) MH-S cell mitochondria respiring on pyruate + malate ± 3-NPA generate low levels of ROS. ROS generation was determined as in (A) and (B). Results in (A), (B) and (C) are means ± SEM (n = 4–6).