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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Future Microbiol. 2009 Nov;4(9):1137–1149. doi: 10.2217/fmb.09.76

Figure 3. The motility of a stator-less Bacillus subtilis mutant (ΔABΔPS) expressing either B. subtilis motAB, B. subtilis motPS or Bacillus clausii motAB.

Figure 3

(A) Motility in a soft agar plate assay. Fresh cells were inoculated into Luria-Bertani medium (pH 7.0) soft agar and incubated at 37°C for 16 h. (B–D) Motility in liquid medium. BS-AB (B), BS-PS (C) and BCl-AB (D) cells were grown at 37°C in Luria-Bertani medium (pH 7.0) and the swimming speed was measured at various concentrations of sodium in 1/20 Tryptone Yeast Extract (TY) medium. 1/20 TY medium contained 0–1 mM sodium. The pH values of 1/20 TY medium used in the swimming speed assays of B. subtilis strains are indicated by the key.

Bcl-AB: B. clausii motAB; BS-AB: B. subtilis motAB; BS-PS: B. subtilis motPS; ΔABΔPS: Deletion strain of the motAB and motPS genes.

Reproduced with permission from [11].