Figure 2.
Phosphorylation of AS160 in rat epitrochlearis samples that were immunoprecipitated with phospho-Akt substrate (PAS) antibody prior to immunoblotting with the anti-AS160 antibody. *p < 0.05 versus Sustained Insulin group. Data are means ± SEM. n=6–8 muscles per treatment at each time. The values on the x-axis refer to the duration (min) after muscles in the Transient Insulin group were removed from insulin prior to being freeze-clamped. Inset: Delta PAS-AS160 for the Transient Insulin Group expressed as a percentage of the Sustained Insulin Group (see Figure 1).