Fig. 4.
Restimulation of splenocytes with LCCWE. Splenocytes harvested 7 days post injection with LCCWE and stimulated in vitro with LCCWE for 72 hours. Supernatants were tested by ELISA for IFN-γ production. A) WT splenocytes harvested from mice injected with either LCCWE or PBS control. B) IFN-γ production inRAG1−/− and WT splenocytes. C) B-cell null splenocytes harvested from mice injected with either LCCWE or PBS control. Data are shown as mean ±SEM n=3. Statistical differences were determined by means of Student’s t test and are represented as * and ** for p<0.05 and p<0.01.