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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Mech Ageing Dev. 2010 Nov 3;132(1-2):8–19. doi: 10.1016/j.mad.2010.10.006

Figure 2.

Figure 2

Polyubiquitination of proteins in the hippocampus. A) Western blot analysis of total protein (20ug/lane) from CA1, CA3, and DG regions of the hippocampi of 8 and 38 mo old rats fed AL and 38 mo old rats on 40% CR diet (n=3 per group) probed with an anti-ubiquitin antibody. Slow migrating poly-ubiquitinated (pUbi) protein substrates are marked by a square bracket. B) Staining against the ubiquitin ligase family member (UBE2J1). GAPDH is shown as a protein loading control. Molecular mass is kDA, on the left.