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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Gene Ther. 2010 Oct 28;18(3):294–303. doi: 10.1038/gt.2010.148

FIGURE 3.

FIGURE 3

Transient expression of OCT4 produces a change in methylation state of the endogenous OCT4 and NANOG promoters in the transfected human skin keratinocytes (HSK-OCT4-GFP). Shown are methylation states of CpG islands −929 to +421 around the transcription start site of the endogenous OCT4 promoter and upstream areas −1091 to −299 of the NANOG promoter for the NTERA-2 cell line (positive control), human skin keratinocytes (HSK-GFP) 48 hours after transient transfection with pcDNA3-GFP (negative control), and human skin keratinocytes (HSK-OCT4-GFP) 48 hours after transient transfection with pcDNA3-OCT4-GFP. Transfected keratinocytes were sorted from untransfected keratinocytes based on GFP; thus the analysis was done only on the transfected cells. Note, demethylation of OCT4 was consistent at +74 and +80, and demethylation of NANOG was consistent at −649, −611, −565, −516, and −378. Numbers relative to translational start site.