| Forward primer | Reverse primer | Tm | bp | |
| hOCT4 | CAGTGCCCGAAACCCACAC | GGAGACCCAGCAGCCTCAAA | 59 | 161 |
| hSOX2 | CAGGAGTTGTCAAGGCAGAGAAGA | GCCGCCGCCGATGATTGTTATTAT | 60 | 320 |
| hNANOG | TTTGGAAGCTGCTGGGGAAG | GATGGGAGGAGGGGAGAGGA | 59 | 194 |
| hREX-1 | CAGATCCTAAACAGCTCGCAGAAT | GCGTACGCAAATTAAAGTCCAGA | 56 | 310 |
| hNeuN | AGCGACAGTTACGGCAGAGT | ACTTGGACTTGGTTGGATGC | 57 | 235 |
| TuJ1 | GGCCAAGTTCTGGGAAGTCA | CGAGTCGCCCACGTAGTTG | 57 | 235 |
| TH | TTGCTGAGATCGCCTTCCAGTACA | AGTACAGCGTGGACAGCTTCTCAA | 60 | 495 |
| Calponin | AGGCTCCGTGAAGAAGATCA | CCACGTTCACCTTGTTTCCT | 55 | 214 |
| SM22α | CGCGAAGTGCAGTCCAAAATCG | GGGCTGGTTCTTCTTCAATGGGG | 59 | 906 |
| K14 | TTCTGAACGAGATGCGTGAC | GCAGCTCAATCTCCAGGTTC | 59 | 188 |
| GAPDH | GAAGGTGAAGGTCGGAGTC | GAAGATGGTGATGGGATTTC | 55 | 226 |