Table 1.
The adependence of the transition temperature on pH and concentration for ELPs with fixed lengths and guest residues.
| ELP guest residues |
Length, L (pentamers) |
pKa |
Trefpro (°C) |
Trefdepro (°C) |
bpro (°C / Ln [µM]) |
bdepro (°C / Ln [µM]) |
R2 | n |
|---|---|---|---|---|---|---|---|---|
|
bV:I:E [1:3:1] |
40 | 5.50 (0.05) | 35.2 (1.8) | 148 (11) | 2.48 (0.53) | 15.5 (2.4) | 0.990 | 21 |
| 80 | 5.51 (0.05) | 21.6 (2.3) | 81.0 (2.0) | 0.42 (0.67) | 5.04 (0.54) | 0.985 | 36 | |
| 160 | 5.29 (0.03) | 18.6 (1.1) | 73.9 (0.8) | 0.28 (0.32) | 5.09 (0.23) | 0.997 | 30 | |
|
cV:H:G:A [1:2:1:1] |
40 | 6.59 (0.09) | 137 (12) | 103 (4) | 8.4 (2.0) | 11.8 (0.9) | 0.979 | 20 |
| 60 | 6.26 (0.06) | 116 (6.7) | 62.1 (2.8) | 4.3 (1.1) | 6.3 (0.6) | 0.978 | 33 | |
| 100 | 6.25 (0.05) | 98.0 (4.6) | 38.5 (2.9) | 2.9 (1.0) | 3.1 (0.7) | 0.982 | 30 | |
| 120 | 6.22 (0.05) | 95.1 (4.4) | 33.4 (2.8) | 2.6 (1.0) | 2.8 (0.7) | 0.985 | 30 | |
a
data fit to Eq 7 and parameters reported as the estimate (standard error).
b
acidic ELP at pH 2.5–7.8, and concentrations from 5–100 µM.
c
basic ELP at pH 5.5–8.0, and concentrations from 10–500 µM.