Skip to main content
. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Free Radic Biol Med. 2010 Dec 30;50(5):624–632. doi: 10.1016/j.freeradbiomed.2010.12.032

Fig. 5. Overexpression of PTEN partially prevents Cd-induced ROS and activation of Akt/mTOR pathway, as well as cell death.

Fig. 5

Indicated cells, infected with Ad-PTEN or Ad-GFP (as control), were exposed with Cd (0–20 μM) for 24 h (A–D), followed by Western blotting using indicated antibodies (A, D), cell viability evaluation using one solution assay (B), or ROS assay (C). For (A) and (D), β-tubulin served as a loading control. Similar results were observed in at least three independent experiments. For (B) and (C), results are presented as mean ± S.E. n=6. aP<0.05, bP<0.01, difference vs. control group.