Table 1.

Effect of acamprosate treatment in the NAc

Metabolites	Wild-type		ENT1–/–
	Saline	Acamprosate	Saline	Acamprosate
N-acetylaspartatic acid (NAA)	0.76 ± 0.03 (12)	0.77 ± 0.03 (10)	0.87 ± 0.02* (12)	0.77 ± 0.04 (12)
Glutamate (Glu)	0.89 ± 0.03 (10)	0.94 ± 0.03 (10)	0.98 ± 0.03* (10)	0.92 ± 0.03 (10)
Glutamine (Gln)	0.40 ± 0.01 (10)	0.39 ± 0.02 (10)	0.45 ± 0.01* (10)	0.37 ± 0.02† (10)
Glutamate+Glutamine (Glx)	1.26 ± 0.04 (10)	1.33 ± 0.04 (10)	1.43 ± 0.04* (10)	1.29 ± 0.05† (10)
Glycerophosphocholine+Phosphocholine (GPC+PCh)	0.34 ± 0.01 (12)	0.33 ± 0.01 (10)	0.35 ± 0.01 (12)	0.31 ± 0.01† (12)
Taurine (Tau)	1.22 ± 0.03 (10)	1.23 ± 0.06 (10)	1.21 ± 0.03 (12)	1.41 ± 0.06*,† (11)
N-acetylaspartylglutamic acid (NAAG)	0.17 ± 0.02 (10)	0.12 ± 0.01 (4)	0.15 ± 0.01 (6)	0.16 ± 0.01 (7)
Alanine (Ala)	0.26 ± 0.04 (8)	0.19 ± 0.01 (7)	0.21 ± 0.02 (9)	0.21 ± 0.02 (9)
γ-aminobutyric acid (GABA)	0.62 ± 0.04 (12)	0.61 ± 0.04 (10)	0.68 ± 0.03 (12)	0.65 ± 0.04 (12)
Guanosine (Gua)	0.59 ± 0.04 (11)	0.57 ± 0.03 (10)	0.53 ± 0.02 (12)	0.56 ± 0.03 (11)
Lactate (Lac)	0.40 ± 0.03 (10)	0.48 ± 0.11 (7)	0.49 ± 0.06 (10)	0.46 ± 0.04 (6)
myo-Inositol (Ins)	1.08 ± 0.05 (12)	0.97 ± 0.10 (10)	1.12 ± 0.04 (12)	1.02 ± 0.03 (12)
Phosphocreatine (PCr)	0.84 ± 0.04 (12)	0.87 ± 0.05 (10)	0.77 ± 0.03 (11)	0.84 ± 0.04 (12)

^*p < 0.05 compared to wild-type mice within each treatment group by two-tailed t-test.

^†p < 0.05 compared to saline treated group within each genotype by two-tailed t-test.

Metabolites are measured relative to total creatine. All data are expressed as mean ± SEM. Number in parentheses is number of mice used in statistical analyses based on Cramér-Rao lower bounds. See “Materials and Methods” for detailed inclusion criteria for statistical analyses.