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Use of TNP-AMP in determination of Kd values for PAP. (A) Binding of TNP-AMP to rSULT1A1 as indicated by an increase in fluorescence intensity at 545 nm (excitation at 435 nm). (B) Displacement of TNP-AMP from the enzyme upon titration with PAP as indicated by the absolute value of the decrease in fluorescence at 545 nm.
