Figure 4.

Neutralization of Endogenous EPO Reduces Bacterial Loads and Stimulates Antibacterial Immune Effector Pathways

(A and B) C57BL/6 mice were infected i.p. with 500 CFU of S. typhimurium and treated with a neutralizing EPO antibody (α-EPO) or isotype control on days 1 and 2 after infection. Bacterial loads were determined in livers (A) and spleens (B) on day 4 after infection.

Spleen samples of PBS-treated controls (n = 4-6 per group) and of Salmonella-infected mice (n = 10 per group) were used to study the expression of immune response genes (C) and NF-κB p65 binding activity (D).

(E) For analysis of survival, C57BL/6 mice were infected i.p. with 500 CFU of S. typhimurium and treated with a neutralizing EPO antibody (or isotype control) every other day starting 2 days after infection as indicated by arrows.