Fig. 7.

Representative micrographs captured from different fields of view of the distribution of (a, b, c) F-actin, (d) Nanog and (e) Oct4 in embryonic stem (ES) cells levitated in the trap for 5 and 60 min at 0.85 MP. (a) Striking stress fibres (white arrows) and focal spots (grey arrows) were observed in single ES cells. Scale bar is 5μm. (b) Some ES cells formed embryoid bodies (EBs) with extensive cell-cell contacts seen through staining of the F-actin cytoskeleton. Scale bar is 50 μm. (c) Close-up of the F-actin staining in EBs shown in (b) accumulated at sites of cell-cell contact (arrows). Scale bar is 5 μm. (d) and (e) Triple-staining images showing the cytoplasmic distribution of Oct4 (d) and (e) Nanog (AlexaFluor Cy3-red dye), Filamentous (F-) actin (Phalloidin 488-green dye) and nucleus (DAPI-blue dye). Scale bar is 10 μm.