Table 2.

Primer sequences for mouse ES pluripotency, early and late differentiation genes

Gene	Oligo	Sequence	Product size (bp)
Nanog	Forward	aaaccaaaggatgaagtgcaa	141
	Reverse	gatgcgttcaccagatagcc
Oct4	Forward	atcactcacatcgccaatca	139
	Reverse	ggaaaggtgtccctgtagcc
Rex1	Forward	ctgggtacgagtggcagttt	117
	Reverse	acgtgtcccagctcttagtcc
Nestin	Forward	ccgcttccgctgggtcactgt	227
	Reverse	ctgagcagctggttctgctcct
Brachyury	Forward	catgtactctttcttgctgg	162
	Reverse	ggtctcgggaaagcagtggc
Mash1	Forward	ccacggtctttgcttctgttt	266
	Reverse	tggggatggcagttgtaaga
Gsc	Forward	cagatgctgccctacatgaac	157
	Reverse	tctgggtacttcgtctcctgg
Fgf5	Forward	tgtgtctcaggggattgtagg	136
	Reverse	agctgttttcttggaatctctcc
Kdr	Forward	tttggcaaatacaacccttcaga	112
	Reverse	gcagaagatactgtcaccacc
Nodal	Forward	ttcaagcctgttgggctctac	312
	Reverse	tccggtcacgtccacatctt
Gfap	Forward	aaaaccgcatcaccattcct	172
	Reverse	acgtccttgtgctcctgctt
Dcx	Forward	ggccaagagtttctgccaag	244
	Reverse	taatgcagggatcagggaca
Otx2	Forward	aaggagccatgttggactgaa	184
	Reverse	gcctgggaatacaggagcag
Pax6	Forward	ggtccatcaaccagcaacct	212
	Reverse	acaccggatcacctctgctt
Mitf	Forward	gagaaatggcggttagaagca	241
	Reverse	caaccacatgagcaacacaga
Nrl	Forward	gatggacgatgccctctcac	258
	Reverse	ctgggctactgataaagcacgaa
Gapdh	Forward	caggttgtctcctgcgactt	127
	Reverse	tgctgtagccgtattcattgtc
β-actin	Forward	ccaccatgtacccaggcatt	141
	Reverse	acagtgaggccaggatggag

ES = embryonic stem.