FIGURE 4.

Diuron increases CYP1A1 mRNA levels in mouse hepatoma cells. Hepa1c1c7 cells were incubated with DMSO (1%, final concentration), TCDD (1 nM), or diuron (2 μM) for 3.5 h at 37°C, mRNA was extracted, subjected to RT-PCR and the resulting products were visualized by agarose gel electrophoresis. PCR amplification of S15 from the same sample was included as the loading control.