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. Author manuscript; available in PMC: 2012 Jan 30.
Published in final edited form as: J Neurosci Methods. 2010 Nov 24;195(1):75–77. doi: 10.1016/j.jneumeth.2010.10.021

Figure 1. Sciatic nerve schwannoma model.

Figure 1

(A) Human schwannoma cells (HEI-193) stably expressing Fluc and mCherry were injected (in 1 µl of culturing medium) into the sciatic nerve of nude mice. In vivo tumor progression was monitored weekly using bioluminescence for 70 days. Bioluminescent images from 15 mice are shown with a pseudocolor bar to indicate degree of bioluminescence. (B) The HEI-193 cells were injected using a glass micropitette (left panel showing pipette next to an un-injected sciatic nerve for size comparison); sciatic nerves containing HEI-193-FC-derived tumors were imaged in vivo 2 months after HEI-193-FC implantation (right panel demonstrating altered gross morphology of sciatic nerve; left panal showing naïve sciatic nerve for comparison); images were acquired through a dissecting stereo-microscope. Tumor-impanted nerves were then removed for histologic analysis. (C) Sections were stained for S100 (FITC) and fluorescent microscopy performed to assess co-localization with mCherry. (D). Hematoxylin and eosin staining of longitudinal sections of the sciatic nerve post-implantation of HEI-193.