Figure 2.

Effect of neutrophil depletion on DC influx to the lung in response to Aspergillus. Mice with Ab-mediated neutrophil depletion and mice treated with isotype control Ab were challenged with killed Aspergillus hyphae 24 hours before i.v. administration of FITC-labeled latex beads. (A) Representative flow cytometry plots and gating strategy of whole lung single cell suspensions gated on CD45⁺ cells, stained for CD11b and CD11c. The bottom flow cytometry plots show the same populations gated on FITC⁺ beads. (B–D) Bead-associated cell subsets in the lungs were identified based on surface expression of CD11b and CD11c. Day 0 represents unchallenged mice. Data shown represent mean ± SEM; n = 5 mice per group per time point. *, p < 0.05 comparing trend between neutropenic and non-neutropenic challenged mice. (E) Bone marrow-derived DCs were labeled with CFSE and transferred intravenously into mice with Ab-mediated neutrophil depletion and mice treated with isotype control Ab on day 2 after challenge with killed Apergillus hyphae. Labeled cells were identified and enumerated in the lung after 4 hours by flow cytometry. n=8 mice per group per time point, pooled results of 2 experiments. *, p = 0.038 compared to the non-neutropenic group.