Figure 5.
Effect of TNF neutralization on lung chemokine levels and lung inflammatory DC number in response to Aspergillus. Mice with Ab-mediated neutrophil depletion and mice treated with isotype control Ab were challenged with killed Aspergillus hyphae. (A–B) Protein levels of CCL2 and CCL20 in whole lung homogenates were measured 1 day after hyphae challenge. (C) Number of lung inflammatory DC in whole lung single cell suspensions on day 3 after hyphal challenge. *, p < 0.05 compared with unchallenged animals; **, p < 0.05 compared with animals receiving control IgG and challenged mice not receiving any treatment. Data shown represent mean ± SEM; n = 5–6 for each group at each time point, representative data from 2 experiments.