Table 4. Nearest-Neighbor Free Energy Parameters for Multibranch Loop Initiation at 37 °C.

model	parameter	value (kcal/mol)	error (kcal/mol)	p-value
eq 24 universal model	aG	6.39	0.86	3.48 × 10−10
	bG	−0.14	0.06	0.0216
	cG	0.25	0.22	0.2659
	dG	1.05	0.17	7.23 × 10−8
	ΔG°37,strain	2.69	0.38	1.88 × 10−9
		R2 = 0.6007, p-value = 3.523 × 10−12
eq 25 model for three-way MBL	aG′	8.06	0.39	<2 × 10−16
	bG′	−0.28	0.06	2.75 × 10−5
	dG′	0.83	0.17	1.57 × 10−5
	ΔG°37,strain′	1.94	0.38	5.32 × 10−6
		R2 = 0.7083, p = 3.737 × 10−13
eq 26 model for four-way MBL	aG′′	6.48	0.45	9.97 × 10−10
	dG′′	1.36	0.34	0.0015
		R2 = 0.5248, p = 0.001503