Table 2.

Oligonucleotide sequences for EMSA

Name of Oligos	Sequence
HTR5A_A_F	CAGAAACTCCCCCACTGGCCAGAGGTTGCAAACATCCGGAT
HTR5A_A_R	ATCCGGATGTTTGCAACCTCTGGCCAGTGGGGGAGTTTCTG
HTR5A_G_F	CAGAAACTCCCCCACTGGCCGGAGGTTGCAAACATCCGGAT
HTR5A_G_R	ATCCGGATGTTTGCAACCTCCGGCCAGTGGGGGAGTTTCTG

Electrophoretic mobility shift assay (EMSA) probes are all 41-mer with the 21st nucleotide carrying the alternative allele (A or G on the forward strand, bold) of rs3734967, the TG-associated promoter single nucleotide polymorphism (SNP) of HTR5A.