Figure 2.

R21A provides better protection against apoptosis. Apoptosis was induced in αA-crystallin overexpressing Chinese hamster ovary (CHO) cells and HeLa cells by either 100 nM staurosporine or 10 μM etoposide. CHO cells were grown to 100% confluency and then treated with 100 nM staurosporine (a) or 10 μM etoposide (b) in 0.01% dimethyl sulfoxide (DMSO) for 36 h. Similarly HeLa cells were treated with either 100 nM staurosporine (c) or 10 μM etoposide (d) for 24 h. Cells were treated with Hoechst stain to estimate the percentages of apoptotic cells. Bars that do not share a common superscript are statistically significant at P<0.0001. In each case, n=3