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Effets of tBid binding to GUVs containing CL. GUVs constituted of PC only (panels a, b, e and f) or of PC/CL 90 : 10 (c, d, g and h) were studied. The GUVs were treated or not treated with 10 nM tBid. (a–d) Light-scattering properties of the GUVs (FSC/SCC). The percentage of vesicles in each gated population is shown. (e–h) tBid-Bodipy Alexa647 versus SSC. The values reported are the mean SSC versus the mean Alexa fluorescence of the selected populations (a–d)
