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. 2010 Dec 2;1(12):e103. doi: 10.1038/cddis.2010.81

Figure 3.

Figure 3

Flow cytometry analysis of caspase-8 binding to GUVs containing CL. FITC-VAD-fmk (500 nM), a fluorescent marker of the caspase-8 active site, was used to stain caspase-8 bound to the vesicles. (a) PC-only-GUV control (autofluorescence at 488 nm excitation). (b) Binding of caspase-8 to PC-only-GUVs: red line, in the absence of z-VAD-fmk (non-fluorescent); black line, following pre-incubation with 5 μM z-VAD-fmk. (c) CL-GUV (composition 90 : 10 PC/CL) control (autofluorescence). (d) Binding of caspase-8 to GUVs containing CL (90 : 10 PC/CL): blue line, in the absence of z-VAD-fmk; black line, after preincubation with 5 μM z-VAD-fmk. Reported values are mean fluorescence and S.D.s for 2500 events involving GUVs over 10 μm in diameter (n=11)