Figure 4.

MEFs at passage 6 contain fragmented centrosomes. Cells were stained for NEDD1 (green), GT335 (red) and Hoechst 33342 (blue), at passages 1 and 6. The centrosomes are enlarged in the insets. Where not obvious, the area enlarged is shown by a smaller box. (a and b) At passage 1 (p1), most cells display normal numbers of centrosomal structures with either 1 (panel a) or 2 (panel b) pairs of centrioles costaining with NEDD1 and GT335. Each pair of centrioles normally appears as a single dot at this resolution. (c–e) By passage 6 (p6), some cells displaying more than two centrosomal structures have complete colocalization of NEDD1 and GT335 in all centriole-like dots (panels c and d), and other cells have some NEDD1-positive but GT335-negative dots (arrows, panel e). The dots are often smaller in centrosomal structures at p6. Scale bars=20 μm