Figure 2.

Gal-3 knockdown enhanced cisplatin-induced activation of caspase-3 and -9. Cells were treated with 50 μM cisplatin for indicated times. Western blot analysis of the expression levels of active caspase-3 (ai) and caspase-9 (bi). Numbers represent the relative intensity of active caspase-3 normalized to β-actin. The values of VC cells were set as ‘1'. (aii) caspase-3 activity assay and (bii) caspase-9 activity assay. Columns (n=3), mean; bars, ±S.D. ^*P<0.05; ^**P<0.01; ^***P<0.001 compared with VC cells. (c) Western blot analysis of Gal-3 expression. Data are representative of three independent experiments