Figure 5.

Gal-3 overexpression reduced cisplatin-induced calpain activation in LNCaP cells. Cells were treated with 50 μM cisplatin for indicated times. (a) The expression levels of Gal-3 in control LNCaP and Gal-3 overexpressing clones. (b) Western blot analysis showed that cisplatin had no effect on the expression level of calpain 2. (c) Calpain activity as represented by western blot analysis of spectrin 150 kD fragment. (d) Casein zymography to detect calpain activity. Purified recombinant rat calpain 2 was used as the positive control. Numbers represent the intensity of calpain 2. The value of VC cells was set as “1”. VC, cells transfected with control vector; #29–11 and #29–23, two Gal-3 overexpressing clones. Data are representative of three independent experiments